Dataset Information

Spatiotemporal Expression and Substrate Specificity Analysis of the Cucumber SWEET Gene Family.

ABSTRACT: The functions of SWEET (Sugar Will Eventually be Exported Transporter) proteins have been studied in a number of crops, but little is known about their roles in cucumber (Cucumis sativus L.), a model plant for studying stachyose metabolism and phloem function. Here, we identified 17 cucumber SWEET genes (CsSWEETs), located on chromosomes 1-6, and classified them into four clades. Two genes from each clade were selected for spatiotemporal expression, subcellular localization, and substrate specificity analyses. Clade I and II proteins were all hexose transporters and targeted to the plasma membrane, while clade III proteins also localized to the plasma membrane, but used sucrose as a substrate. Clade IV SWEET proteins were localized to the tonoplast, and used hexose as a substrate. The eight tested CsSWEET genes were most highly expressed in flower, which represents a large sink in plants. However, each gene also showed specific expression patterns: three of the eight tested genes were highly expressed in mature leaves, two in roots, two in fruit, two in stems, and one was detected in all tested organs. The likely biological roles of each are discussed based on the above results.

SUBMITTER: Li Y

PROVIDER: S-EPMC5664084 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Spatiotemporal Expression and Substrate Specificity Analysis of the Cucumber SWEET Gene Family.

Li Yaxin Y Feng Sheng S Ma Si S Sui Xiaolei X Zhang Zhenxian Z

Frontiers in plant science 20171027

The functions of SWEET (Sugar Will Eventually be Exported Transporter) proteins have been studied in a number of crops, but little is known about their roles in cucumber (Cucumis sativus L.), a model plant for studying stachyose metabolism and phloem function. Here, we identified 17 cucumber SWEET genes (CsSWEETs), located on chromosomes 1-6, and classified them into four clades. Two genes from each clade were selected for spatiotemporal expression, subcellular localization, ...[more]

PMID: 29163584

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Project description:Sweet cherry (Prunus avium) is one of the economically valuable horticultural fruit trees and it is widely cultivated throughout the world. Whirly (WHY) genes are a unique gene family with few members and have important biological functions in plant growth, development, and response to abiotic stress. This study utilized whole-genome identification to conduct a comprehensive analysis of the WHY genes in sweet cherry and examined their transcription levels in different tissues and under abiotic stress to explore their functions. Two WHY genes were identified in the sweet cherry genome and named PavWHY1 and PavWHY2, respectively, based on their homology with those in Arabidopsis thaliana. Both genes have theoretical isoelectric points greater than seven and are hydrophilic proteins, suggesting that they may be localized in plastids. The two genes are evolutionarily classified into two categories, with large differences in gene structure, and highly similar protein tertiary structures, and both have conserved domains of WHY. PavWHY1 and PavWHY2 are collinear with AtWHY1 and AtWHY2, respectively. The promoter sequence contains cis-acting elements related to hormones and abiotic stress, which are differentially expressed during flower bud differentiation, fruit development, and cold accumulation. qRT-PCR showed that PavWHY1 and PavWHY2 were differentially expressed in flower and fruit development and responded to low temperature and exogenous ABA treatment. The recombinant plasmid pGreenII-0800-Luc with the promoters of these two genes can activate luciferase expression in tobacco. Protein interaction predictions indicate that these gene products may interact with other proteins. This study reveals the molecular features, evolutionary relationships, and expression patterns of sweet cherry WHY genes, and investigates the activities of their promoters, which lays the foundation for further exploration of their biological functions and provides new insights into the WHY gene family in Rosaceae.

Dataset Information

Spatiotemporal Expression and Substrate Specificity Analysis of the Cucumber SWEET Gene Family.

Publications

Spatiotemporal Expression and Substrate Specificity Analysis of the Cucumber <i>SWEET</i> Gene Family.

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