⁸⁹Zr-Cobalamin PET Tracer: Synthesis, Cellular Uptake, and Use for Tumor Imaging.

ABSTRACT: Vitamin B₁₂, or cobalamin (Cbl), is an essential nutrient. Acquisition, transport, and cellular internalization of Cbl are dependent on specific binding proteins and associated receptors. The circulating transport protein transcobalamin (TC) promotes cellular uptake via binding to specific receptors such as CD320, a receptor upregulated in several cancer cell lines. In this study, we report the successful synthesis of ⁸⁹Zirconium-labeled Cbl that was derivatized with desferrioxamine (⁸⁹Zr-Cbl). We document the purity of the tracer and its binding to TC compared with that of unmodified cyano-Cbl (CN-Cbl). In vitro studies employing the CD320 receptor-positive breast cancer cell line MDA-MB-453 showed a 6- to 10-fold greater uptake of ⁸⁹Zr-Cbl when

SUBMITTER: Kuda-Wedagedara ANW 

PROVIDER: S-EPMC5664145 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature