The influence of microRNAs and poly(A) tail length on endogenous mRNA-protein complexes.

ABSTRACT:

Background

All mRNAs are bound in vivo by proteins to form mRNA-protein complexes (mRNPs), but changes in the composition of mRNPs during posttranscriptional regulation remain largely unexplored. Here, we have analyzed, on a transcriptome-wide scale, how microRNA-mediated repression modulates the associations of the core mRNP components eIF4E, eIF4G, and PABP and of the decay factor DDX6 in human cells.

Results

Despite the transient nature of repressed intermediates, we detect significant changes in mRNP composition, marked by dissociation of eIF4G and PABP, and by recruitment of DDX6. Furthermore, although poly(A)-tail length has been considered critical in post-transcriptional regulation, differences in steady-state tail length explain little of the variation in either PAB

SUBMITTER: Rissland OS 

PROVIDER: S-EPMC5664449 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature