Staufen1 inhibits MyoD translation to actively maintain muscle stem cell quiescence.

ABSTRACT: Tissue regeneration depends on the timely activation of adult stem cells. In skeletal muscle, the adult stem cells maintain a quiescent state and proliferate upon injury. We show that muscle stem cells (MuSCs) use direct translational repression to maintain the quiescent state. High-resolution single-molecule and single-cell analyses demonstrate that quiescent MuSCs express high levels of Myogenic Differentiation 1 (MyoD) transcript in vivo, whereas MyoD protein is absent. RNA pulldowns and costainings show that MyoD mRNA interacts with Staufen1, a potent regulator of mRNA localization, translation, and stability. Staufen1 prevents MyoD translation through its interaction with the MyoD 3'-UTR. MuSCs from Staufen1 heterozygous (Staufen1^+/-) mice have increased MyoD protein expres

SUBMITTER: de Morree A 

PROVIDER: S-EPMC5664522 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature