MiR-422a inhibits cell proliferation in colorectal cancer by targeting AKT1 and MAPK1.

ABSTRACT:

Background

miRNAs are regarded as molecular biomarkers and therapeutic targets for colorectal cancer (CRC), a series of miRNAs have been proven to involve into CRC carcinogenesis, invasion and metastasis. Aberrant miR-422a expression and its roles have been reported in some cancers. However, the function and underlying mechanism of miR-422a in the progression of CRC remain largely unknown.

Methods

Real-time PCR were used to quantify miR-422a expression in CRC tissues. Both vivo and vitro functional assays showed miR-422a inhibits CRC cell proliferation. Target prediction program (miRBase) and luciferase reporter assays were conducted to confirm the target genes AKT1 and MAPK1 of miR-422a. Specimens from 50 patients with CRC were analyzed for the correlation between the expre

SUBMITTER: Wei WT 

PROVIDER: S-EPMC5664829 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature