Isolation and characterization of microsatellite markers for Hypochaeris incana (Asteraceae) and close relatives.

ABSTRACT:

Premise of the study

We developed microsatellite markers to study clonal growth and interspecific hybridization in the Patagonian and subantarctic plant Hypochaeris incana (Asteraceae) and its closest relatives.

Methods and results

We developed primers for microsatellite loci from 454 sequence reads of genomic DNA of H. incana. We tested them on individuals of H. acaulis, H. hookeri, H. incana, H. palustris, and H. tenuifolia. We selected 15 polymorphic microsatellite loci, which delivered clearly scorable fragments in most or all species. With mean values between 0.7 and 0.8, the expected heterozygosity in populations of H. incana is high.

Conclusions

Due to high levels of polymorphism, the developed markers mak

SUBMITTER: Wang P 

PROVIDER: S-EPMC5664967 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature