Development and characterization of genomic SSR markers for Anneslea fragrans (Pentaphylacaceae).

ABSTRACT:

Premise of the study

The genus Anneslea (Pentaphylacaceae) contains four species and six varieties, most of which are locally endemic. Here, simple sequence repeat (SSR) markers were developed for the conservation of these species.

Methods and results

The genome of A. fragrans was sequenced and de novo assembled into 445,162 contigs, of which 30,409 SSR loci were detected. Primers for 100 SSR loci were validated with PCR amplification in three populations of A. fragrans. Seventy-nine loci successfully amplified, and 30 were polymorphic. The mean number of alleles, observed heterozygosity, and expected heterozygosity were 7.01 ± 1.60, 0.817 ± 0.241, and 0.796 ± 0.145, respectively. Most primers could be amplified in Ternstroemia gymnanthera, T.

SUBMITTER: Sun L 

PROVIDER: S-EPMC5664968 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature