Identification of Equid herpesvirus 2 in tissue-engineered equine tendon.

ABSTRACT: Background: Incidental findings of virus-like particles were identified following electron microscopy of tissue-engineered tendon constructs (TETC) derived from equine tenocytes. We set out to determine the nature of these particles, as there are few studies which identify virus in tendons per se, and their presence could have implications for tissue-engineering using allogenic grafts. Methods: Virus particles were identified in electron microscopy of TETCs. Virion morphology was used to initially hypothesise the virus identity.  Next generation sequencing was implemented to identify the virus. A pan herpesvirus PCR was used to validate the RNASeq findings using an independent platform. Histological analysis and biochemical analysis was undertaken on the TETCs. Resu

SUBMITTER: Wardle R 

PROVIDER: S-EPMC5664983 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature