Tuning cell adhesive properties via layer-by-layer assembly of chitosan and alginate.

ABSTRACT: Understanding the mechanisms controlling cell-multilayer film interactions is crucial to the successful engineering of these coatings for biotechnological and biomedical applications. Herein, we present a strategy to tune the cell adhesive properties of multilayers based on marine polysaccharides with and without cross-linking and/or coating with extracellular matrix proteins. Chemical cross-linking of multilayers improved mechanical properties of the coatings but also elicited changes in surface chemistry that alter the adhesion of human umbilical vein endothelial cells. We evaluated a strategy to decouple the mechanical and chemical properties of these films, enabling the transition from cell-adhesive to cell-resistant multilayers. Addition of chitosan/alginate multilayers on top of cros

SUBMITTER: Silva JM 

PROVIDER: S-EPMC5665021 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature