Distinctive features of tumor-infiltrating ?? T lymphocytes in human colorectal cancer.

ABSTRACT: ?? T cells usually infiltrate many different types of cancer, but it is unclear whether they inhibit or promote tumor progression. Moreover, properties of tumor-infiltrating ?? T cells and those in the corresponding normal tissue remain largely unknown. Here we have studied features of ?? T cells in colorectal cancer, normal colon tissue and peripheral blood, and correlated their levels with clinicopathologic hallmarks. Flow cytometry and transcriptome analyses showed that the tumor comprised a highly variable rate of TILs (5-90%) and 4% ?? T cells on average, with the majority expressing V?1. Most V?1 and V?2 T cells showed a predominant effector memory phenotype and had reduced production of IFN- ? which was likely due to yet unidentified inhibitory molecules present in cancer stem cell secretome. Transcriptome analyses revealed that patients containing abundant ?? T cells had significantly longer 5-year disease free survival rate, suggesting their efficacy in controlling tumor at very early stage.

SUBMITTER: Meraviglia S 

PROVIDER: S-EPMC5665062 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature