Spatiotemporally Non-Uniform Ca²⁺ Dynamics of Cardiac Purkinje Fibers in Mouse Myocardial Infarct.

ABSTRACT: Surviving Purkinje fibers in myocardial infarct are regarded as an important substrate in arrhythmogenesis. However, poorly understood are functional properties of Purkinje fibers in the infarcted heart. We sought to visualize intracellular Ca²⁺ ([Ca²⁺]_i) dynamics of Purkinje fiber networks in the mouse myocardial infarct. Using 3- to 4-day-old or 7- to 9-day-old infarcted hearts after the left coronary-artery ligation corresponding, respectively, to acute or healing phase, we conducted rapid fluo4-fluorescence imaging on the endocardial surface of the left ventricular septum by macro-zoom fluorescence microscopy and rapid-scanning confocal microscopy. In contrast with the intact heart, where uniform Ca²⁺ transients propagated rapidly, the infarc

SUBMITTER: Matsuyama TA 

PROVIDER: S-EPMC5665107 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature