Myokine Expression in Muscle and Myotubes in Response to Exercise Stimulation.

ABSTRACT:

Purpose

Myokines have been shown to affect muscle physiology and exert systemic effects. We endeavored to investigate a panel of myokine mRNA expression after a single exercise bout (studies 1 and 2) to measure myokine mRNA in primary human myotubes in an in vitro exercise model (study 2).

Methods

Vastus lateralis muscle biopsies were obtained from 20 healthy males (age, 24.0 ± 4.5 yr; BMI, 23.6 ± 1.8 kg·m)(-2) before and after a single exercise bout (650 kcal at 50% V˙O2max). Primary myotubes from active and sedentary male donors were treated with a pharmacological cocktail (palmitate, forskolin, and ionomycin (PFI)) to mimic exercise-stimulated contractions in vitro.

Results

Interleukin 6 and 8 (IL-6 and IL-8), leukocyte-inducing factor, and connective tissue growth

SUBMITTER: Covington JD 

PROVIDER: S-EPMC5665363 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature