De novo transcriptome sequencing and assembly from apomictic and sexual Eragrostis curvula genotypes.

ABSTRACT: A long-standing goal in plant breeding has been the ability to confer apomixis to agriculturally relevant species, which would require a deeper comprehension of the molecular basis of apomictic regulatory mechanisms. Eragrostis curvula (Schrad.) Nees is a perennial grass that includes both sexual and apomictic cytotypes. The availability of a reference transcriptome for this species would constitute a very important tool toward the identification of genes controlling key steps of the apomictic pathway. Here, we used Roche/454 sequencing technologies to generate reads from inflorescences of E. curvula apomictic and sexual genotypes that were de novo assembled into a reference transcriptome. Near 90% of the 49568 assembled isotigs showed sequence similarity to sequences deposited in the publ

SUBMITTER: Garbus I 

PROVIDER: S-EPMC5665505 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature