Rapid regrowth and detection of microbial contaminants in equine fecal microbiome samples.

ABSTRACT: Advances have been made to standardize 16S rRNA gene amplicon based studies for inter-study comparisons, yet there are many opportunities for systematic error that may render these comparisons improper and misleading. The fecal microbiome of horses has been examined previously, however, no universal horse fecal collection method and sample processing procedure has been established. This study was initialized in large part to ensure that samples collected by different individuals from different geographical areas (i.e., crowdsourced) were not contaminated due to less than optimal sampling or holding conditions. In this study, we examined the effect of sampling the surface of fecal pellets compared to homogenized fecal pellets, and also the effect of time of sampling after defecation on 'blo

SUBMITTER: Beckers KF 

PROVIDER: S-EPMC5665523 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature