Fluorinated Methacrylamide Chitosan Hydrogels Enhance Cellular Wound Healing Processes.

ABSTRACT: Low availability of oxygen can lead to stalled wound healing processes and chronic wounds. To address local hypoxia and to better understand direct cellular benefits, a perfluorocarbon conjugated chitosan (MACF) hydrogel that delivers oxygen was created and applied for the first time to in vitro cultures of human dermal fibroblasts and human epidermal keratinocytes under both normoxic (21% O₂) and hypoxic (1% O₂) environments. Results revealed that local application of MACF provided 233.8 ± 9.9 mmHg oxygen partial pressure at 2 h and maintained equilibrium oxygen levels that were approximately 17 mmHg partial pressure greater than untreated controls. Cell culture experiments showed that MACF oxygenating gels improved cellular functions involved in wound healing such a

SUBMITTER: Akula S 

PROVIDER: S-EPMC5665717 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature