Off-axis electron holography of bacterial cells and magnetic nanoparticles in liquid.

ABSTRACT: The mapping of electrostatic potentials and magnetic fields in liquids using electron holography has been considered to be unrealistic. Here, we show that hydrated cells of Magnetospirillum magneticum strain AMB-1 and assemblies of magnetic nanoparticles can be studied using off-axis electron holography in a fluid cell specimen holder within the transmission electron microscope. Considering that the holographic object and reference wave both pass through liquid, the recorded electron holograms show sufficient interference fringe contrast to permit reconstruction of the phase shift of the electron wave and mapping of the magnetic induction from bacterial magnetite nanocrystals. We assess the challenges of performing in situ magnetization reversal experiments using a fluid cell specimen hold

SUBMITTER: Prozorov T 

PROVIDER: S-EPMC5665829 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature