A Method for Quantifying Molecular Interactions Using Stochastic Modelling and Super-Resolution Microscopy.

ABSTRACT: We introduce the Interaction Factor (IF), a measure for quantifying the interaction of molecular clusters in super-resolution microscopy images. The IF is robust in the sense that it is independent of cluster density, and it only depends on the extent of the pair-wise interaction between different types of molecular clusters in the image. The IF for a single or a collection of images is estimated by first using stochastic modelling where the locations of clusters in the images are repeatedly randomized to estimate the distribution of the overlaps between the clusters in the absence of interaction (IF = 0). Second, an analytical form of the relationship between IF and the overlap (which has the random overlap as its only parameter) is used to estimate the IF for the experimentally observed

SUBMITTER: Bermudez-Hernandez K 

PROVIDER: S-EPMC5665986 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature