Identifying the Active Microbiome Associated with Roots and Rhizosphere Soil of Oilseed Rape.

ABSTRACT: RNA stable isotope probing and high-throughput sequencing were used to characterize the active microbiomes of bacteria and fungi colonizing the roots and rhizosphere soil of oilseed rape to identify taxa assimilating plant-derived carbon following ¹³CO₂ labeling. Root- and rhizosphere soil-associated communities of both bacteria and fungi differed from each other, and there were highly significant differences between their DNA- and RNA-based community profiles. Verrucomicrobia, Proteobacteria, Planctomycetes, Acidobacteria, Gemmatimonadetes, Actinobacteria, and Chloroflexi were the most active bacterial phyla in the rhizosphere soil. Bacteroidetes were more active in roots. The most abundant bacterial genera were

SUBMITTER: Gkarmiri K 

PROVIDER: S-EPMC5666129 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature