Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits.

ABSTRACT:

Objective

The cytokine inducible SH2-containing protein (CISH), which might play a role in porcine intestine immune responses, was one of the promising candidate genes for piglet anti-disease traits. An experiment was conducted to characterize the porcine CISH (pCISH) gene and to evaluate its genetic effects on pig anti-disease breeding.

Methods

Both reverse transcription polymerase chain reaction (RT-PCR) and PCR were performed to obtain the sequence of pCISH gene. A pEGFP-C1-CISH vector was constructed and transfected into PK-15 cells to analysis the distribution of pCISH. The sequences of individuals were compared with each other to find the polymorphisms in pCISH gene. The association analysis was performed in Min pigs and Landrace pigs to evaluate the genetic effects on

SUBMITTER: Niu B 

PROVIDER: S-EPMC5666171 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature