Modeling cancer driver events in vitro using barrier bypass-clonal expansion assays and massively parallel sequencing.

ABSTRACT: The information on candidate cancer driver alterations available from public databases is often descriptive and of limited mechanistic insight, which poses difficulties for reliable distinction between true driver and passenger events. To address this challenge, we performed in-depth analysis of whole-exome sequencing data from cell lines generated by a barrier bypass-clonal expansion (BBCE) protocol. The employed strategy is based on carcinogen-driven immortalization of primary mouse embryonic fibroblasts and recapitulates early steps of cell transformation. Among the mutated genes were almost 200 COSMIC Cancer Gene Census genes, many of which were recurrently affected in the set of 25 immortalized cell lines. The alterations affected pathways regulating DNA damage response and repair, tr

SUBMITTER: Huskova H 

PROVIDER: S-EPMC5666318 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature