Plasmid-based one-pot saturation mutagenesis.

ABSTRACT: Deep mutational scanning is a foundational tool for addressing the functional consequences of large numbers of mutants, but a more efficient and accessible method for construction of user-defined mutagenesis libraries is needed. Here we present nicking mutagenesis, a robust, single-day, one-pot saturation mutagenesis method performed on routinely prepped plasmid dsDNA. The method can be used to produce comprehensive or single- or multi-site saturation mutagenesis libraries.

SUBMITTER: Wrenbeck EE 

PROVIDER: S-EPMC5666567 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature