Imaging B Cells in a Mouse Model of Multiple Sclerosis Using ⁶⁴Cu-Rituximab PET.

ABSTRACT: B lymphocytes are a key pathologic feature of multiple sclerosis (MS) and are becoming an important therapeutic target for this condition. Currently, there is no approved technique to noninvasively visualize B cells in the central nervous system (CNS) to monitor MS disease progression and response to therapies. Here, we evaluated ⁶⁴Cu-rituximab, a radiolabeled antibody specifically targeting the human B cell marker CD20, for its ability to image B cells in a mouse model of MS using PET. Methods: To model CNS infiltration by B cells, experimental autoimmune encephalomyelitis (EAE) was induced in transgenic mice that express human CD20 on B cells. EAE mice were given subcutaneous injections of myelin oligodendrocyte glycoprotein fragment_1-125 emulsified in comple

SUBMITTER: James ML 

PROVIDER: S-EPMC5666646 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature