Selective glycoprotein detection through covalent templating and allosteric click-imprinting.

ABSTRACT: Many glycoproteins are intimately linked to the onset and progression of numerous heritable or acquired diseases of humans, including cancer. Indeed the recognition of specific glycoproteins remains a significant challenge in analytical method and diagnostic development. Herein, a hierarchical bottom-up route exploiting reversible covalent interactions with boronic acids and so-called click chemistry for the fabrication of glycoprotein selective surfaces that surmount current antibody constraints is described. The self-assembled and imprinted surfaces, containing specific glycoprotein molecular recognition nanocavities, confer high binding affinities, nanomolar sensitivity, exceptional glycoprotein specificity and selectivity with as high as 30 fold selectivity for prostate specific antige

SUBMITTER: Stephenson-Brown A 

PROVIDER: S-EPMC5666680 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature