Fluorescence spectroscopy and microscopy as tools for monitoring redox transformations of uranium in biological systems.

ABSTRACT: We report a study of redox reactions of uranium in model conditions using luminescence spectroscopy, which with its ease and wide availability has the potential to offer new insights into a bioremediation strategy of particular interest - the enzymatic reduction of U^VIO₂²⁺ by bacteria such as Geobacter sulfurreducens. The inherent luminescent properties of U^VIO₂²⁺ have been combined with confocal fluorescence microscopy techniques and lifetime image mapping to report directly on uranium concentration, localisation and oxidation state in cellular systems during uranium bioreduction, suggesting that localisation of uranyl species on the cell membrane surface plays an important role and that extracellular biogenic featur

SUBMITTER: Jones DL 

PROVIDER: S-EPMC5666681 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature