Transforming Growth Factor-β Drives the Transendothelial Migration of Hepatocellular Carcinoma Cells.

ABSTRACT: The entry of malignant hepatocytes into blood vessels is a key step in the dissemination and metastasis of hepatocellular carcinoma (HCC). The identification of molecular mechanisms involved in the transmigration of malignant hepatocytes through the endothelial barrier is of high relevance for therapeutic intervention and metastasis prevention. In this study, we employed a model of hepatocellular transmigration that mimics vascular invasion using hepatic sinusoidal endothelial cells and malignant hepatocytes evincing a mesenchymal-like, invasive phenotype by transforming growth factor (TGF)-β. Labelling of respective cell populations with various stable isotopes and subsequent mass spectrometry analyses allowed the "real-time" detection of molecular changes in both transmigrating hepatocyt

SUBMITTER: Koudelkova P 

PROVIDER: S-EPMC5666801 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature