Using low-risk factors to generate non-integrated human induced pluripotent stem cells from urine-derived cells.

ABSTRACT:

Background

Because the lack of an induced pluripotent stem cell (iPSC) induction system with optimal safety and efficiency limits the application of these cells, development of such a system is important.

Methods

To create such an induction system, we screened a variety of reprogrammed plasmid combinations and multiple compounds and then verified the system's feasibility using urine cells from different individuals. We also compared large-scale iPSC chromosomal variations and expression of genes associated with genomic stability between this system and the traditional episomal system using karyotype and quantitative reverse transcription polymerase chain reaction analyses.

Results

We developed a high-efficiency episomal system, the 6F/BM1-4C system, lacking tumorigeni

SUBMITTER: Wang L 

PROVIDER: S-EPMC5667457 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature