Proteomic analysis of filaggrin deficiency identifies molecular signatures characteristic of atopic eczema.

ABSTRACT:

Background

Atopic eczema (AE) is characterized by skin barrier and immune dysfunction. Null mutations in filaggrin (FLG), a key epidermal barrier protein, strongly predispose to AE; however, the precise role of FLG deficiency in AE pathogenesis remains incompletely understood.

Objectives

We sought to identify global proteomic changes downstream of FLG deficiency in human epidermal living skin-equivalent (LSE) models and validate findings in skin of patients with AE.

Methods

Differentially expressed proteins from paired control (nontargeting control short hairpin RNA [shNT]) and FLG knockdown (FLG knockdown short hairpin RNA [shFLG]) LSEs were identified by means of proteomic analysis (liquid chromatography-mass spectrometry) and Ingenuity Pathway Analysis. Expression

SUBMITTER: Elias MS 

PROVIDER: S-EPMC5667587 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature