Nanobodies targeting norovirus capsid reveal functional epitopes and potential mechanisms of neutralization.

ABSTRACT: Norovirus is the leading cause of gastroenteritis worldwide. Despite recent developments in norovirus propagation in cell culture, these viruses are still challenging to grow routinely. Moreover, little is known on how norovirus infects the host cells, except that histo-blood group antigens (HBGAs) are important binding factors for infection and cell entry. Antibodies that bind at the HBGA pocket and block attachment to HBGAs are believed to neutralize the virus. However, additional neutralization epitopes elsewhere on the capsid likely exist and impeding the intrinsic structural dynamics of the capsid could be equally important. In the current study, we investigated a panel of Nanobodies in order to probe functional epitopes that could trigger capsid rearrangement and/ or interfere with H

SUBMITTER: Koromyslova AD 

PROVIDER: S-EPMC5667739 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature