TRPM5 mediates acidic extracellular pH signaling and TRPM5 inhibition reduces spontaneous metastasis in mouse B16-BL6 melanoma cells.

ABSTRACT: Extracellular acidity is a hallmark of solid tumors and is associated with metastasis in the tumor microenvironment. Acidic extracellular pH (pH _e ) has been found to increase intracellular Ca²⁺ and matrix metalloproteinase-9 (MMP-9) expression by activating NF-κB in the mouse B16 melanoma model. The present study assessed whether TRPM5, an intracellular Ca²⁺-dependent monovalent cation channel, is associated with acidic pH _e signaling and induction of MMP-9 expression in this mouse melanoma model. Treatment of B16 cells with Trpm5 siRNA reduced acidic pH _e -induced MMP-9 expression. Enforced expression of Trpm5 increased the rate of acidic pH _e -induced MMP-9 expression, as well as

SUBMITTER: Maeda T 

PROVIDER: S-EPMC5667964 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature