Novel patient-derived xenograft and cell line models for therapeutic testing of pediatric liver cancer.

ABSTRACT:

Background & aims

Pediatric liver cancer is a rare but serious disease whose incidence is rising, and for which the therapeutic options are limited. Development of more targeted, less toxic therapies is hindered by the lack of an experimental animal model that captures the heterogeneity and metastatic capability of these tumors.

Methods

Here we established an orthotopic engraftment technique to model a series of patient-derived tumor xenograft (PDTX) from pediatric liver cancers of all major histologic subtypes: hepatoblastoma, hepatocellular cancer and hepatocellular malignant neoplasm. We utilized standard (immuno) staining methods for histological characterization, RNA sequencing for gene expression profiling and genome sequencing for identification of druggable targets.

SUBMITTER: Bissig-Choisat B 

PROVIDER: S-EPMC5668139 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature