Photobiomodulation leads to enhanced radiosensitivity through induction of apoptosis and autophagy in human cervical cancer cells.

ABSTRACT: The radiomodulatory effect of photobiomodulation (PBM) has recently been studied in cancer cells. The aim of this study was to investigate cellular mechanisms involved in the X-ray radiosensitivity of HeLa cells pre-exposed to PBM. HeLa cells were irradiated with 685 nm laser at different energy densities prior to X-ray ionizing radiation. After irradiation, clonogenic cell survival, cell death due to apoptosis and autophagy were determined. Levels of intracellular reactive oxygen species (ROS), DNA damage and, cell cycle distribution after PBM were measured. PBM at different energy densities (5-20 J/cm² ) was not cytotoxic. However, HeLa cells pre-exposed to 20 J/cm² showed enhanced inhibition of colony formation following ionizing radiation. Enhanced radiosensitivit

SUBMITTER: Djavid GE 

PROVIDER: S-EPMC5668202 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature