Identification of seeds based on molecular markers and secondary metabolites in Senna obtusifolia and Senna occidentalis.

ABSTRACT:

Background

Senna obtusifolia and Senna occidentalis (Leguminosae), whose seeds have similar appearance and chemical constituents, are easily confused in using their seeds. To elucidate the similarities and differences between S. obtusifolia seeds and S. occidentalis seeds, three molecular markers and high performance liquid chromatography (HPLC) were employed to evaluate the seeds characteristics of these two medicinal herbs.

Results

The results showed that selected 3 ISSR and 7 SCoT primers could distinguish S. obtusifolia seeds from S. occidentalis seeds based on the specific band and UPGMA dendrogram. ITS2 sequence indicated that the intra-specific similarity of 20 S. obtusifolia and 16 S. occidentalis was 99.79 and 100.0%, respectively, while the inter-specific similarit

SUBMITTER: Mao R 

PROVIDER: S-EPMC5668216 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature