Intracellular construction of topology-controlled polypeptide nanostructures with diverse biological functions.

ABSTRACT: Topological structures of bio-architectonics and bio-interfaces play major roles in maintaining the normal functions of organs, tissues, extracellular matrix, and cells. In-depth understanding of natural self-assembly mechanisms and mimicking functional structures provide us opportunities to artificially control the natural assemblies and their biofunctions. Here, we report an intracellular enzyme-catalyzed polymerization approach for efficient synthesis of polypeptides and in situ construction of topology-controlled nanostructures. We reveal that the phase behavior and topological structure of polypeptides are encoded in monomeric peptide sequences. Next, we elucidate the relationship between polymerization dynamics and their temperature-dependent topological transition in biological cond

SUBMITTER: Li LL 

PROVIDER: S-EPMC5668255 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature