Freeform micropatterning of living cells into cell culture medium using direct inkjet printing.

ABSTRACT: Microfabrication methods have widely been used to control the local cellular environment on a micron scale. However, accurately mimicking the complexity of the in vivo tissue architecture while maintaining the freedom of form and design is still a challenge when co-culturing multiple types of cells on the same substrate. For the first time, we present a drop-on-demand inkjet printing method to directly pattern living cells into a cell-friendly liquid environment. High-resolution control of cell location is achieved by precisely optimizing printing parameters with high-speed imaging of cell jetting and impacting behaviors. We demonstrated the capabilities of the direct cell printing method by co-printing different cells into various designs, including complex gradient arrangements. Finally,

SUBMITTER: Park JA 

PROVIDER: S-EPMC5668285 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature