A real time metabolomic profiling approach to detecting fish fraud using rapid evaporative ionisation mass spectrometry.

ABSTRACT:

Introduction

Fish fraud detection is mainly carried out using a genomic profiling approach requiring long and complex sample preparations and assay running times. Rapid evaporative ionisation mass spectrometry (REIMS) can circumvent these issues without sacrificing a loss in the quality of results.

Objectives

To demonstrate that REIMS can be used as a fast profiling technique capable of achieving accurate species identification without the need for any sample preparation. Additionally, we wanted to demonstrate that other aspects of fish fraud other than speciation are detectable using REIMS.

Methods

478 samples of five different white fish species were subjected to REIMS analysis using an electrosurgical knife. Each sample was cut 8-12 times with each one lasting 3-5 

SUBMITTER: Black C 

PROVIDER: S-EPMC5668337 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature