Microfluidic preparation of anchored cell membrane sheets for in vitro analyses and manipulation of the cytoplasmic face.

ABSTRACT: Molecular networks on the cytoplasmic faces of cellular plasma membranes are critical research topics in biological sciences and medicinal chemistry. However, the selective permeability of the cell membrane restricts the researchers from accessing to the intact intracellular factors on the membrane from the outside. Here, a microfluidic method to prepare cell membrane sheets was developed as a promising tool for direct examination of the cytoplasmic faces of cell membranes. Mammalian cells immobilized on a poly(ethylene glycol)-lipid coated substrate were rapidly and efficiently fractured, with the sheer stress of laminar flow in microchannels, resulting in isolation of the bottom cell membrane sheets with exposed intact cytoplasmic faces. On these faces of the cell membrane sheets, both l

SUBMITTER: Izuta S 

PROVIDER: S-EPMC5668413 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature