Dual targeting improves capture of ultrasound microbubbles towards activated platelets but yields no additional benefit for imaging of arterial thrombosis.

ABSTRACT: Platelets can be found on the surface of inflamed and ruptured atherosclerotic plaques. Thus, targeting of activated platelets may allow for molecular imaging of vulnerable atherosclerotic lesions. We here investigated microbubbles (MB) functionalized with the selectin ligand sialyl Lewis^a individually (MB_sLea) or dually with sLe^a and an antibody targeting ligand-induced binding sites of the activated GPIIb/IIIa receptor (MB_Dual). Assessed by in vitro flow chamber, targeted MB exhibited increased adhesion to platelets as compared to MB_Control. While MB_sLea rolled slowly on the platelets' surface, MB_Dual enhanced the percentage of firm adhesion. In vivo, MB were investigated by ultrasound in a model of ferric chlor

SUBMITTER: Gunther F 

PROVIDER: S-EPMC5668440 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature