Novel acidic phospholipase A₂ from Porthidium hyoprora causes inflammation with mast cell rich infiltrate.

ABSTRACT: Phospholipases A₂ (PLA₂) are a group of enzymes that hydrolyze phospholipids at the sn-2 position, being present in all nature. In venomous animals, these proteins assume a special role, being able to exert diverse pharmacological effects. In this work, authors identified a new isoform of PLA₂ in the venom of Porthidium hyoprora, which was isolated through sequential chromatographic steps and named PhTX-III. The enzyme was characterized biochemically and structurally. Structural studies using mass spectrometry confirmed an acidic secretory PLA₂, family IIA, with molecular mass of 13,620.9 Da and identification of 86% of its primary sequence. PhTX-III did not exhibit myotoxic, anticoagulant or antibacterial effects, often present in

SUBMITTER: Marques PP 

PROVIDER: S-EPMC5668520 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature