Modulation of mesenteric collecting lymphatic contractions by σ₁-receptor activation and nitric oxide production.

ABSTRACT: Recently, it has been reported that a σ-receptor antagonist could reduce inflammation-induced edema. Lymphatic vessels play an essential role in removing excess interstitial fluid. We tested the hypothesis that activation of σ-receptors would reduce or weaken collecting lymphatic contractions. We used isolated, cannulated rat mesenteric collecting lymphatic vessels to study contractions in response to the σ-receptor agonist afobazole in the absence and presence of different σ-receptor antagonists. We used RT-PCR and Western blot analysis to investigate whether these vessels express the σ₁-receptor and immunofluorescence confocal microscopy to examine localization of the σ₁-receptor in the collecting lymphatic wall. Using N-nitro-l-arginine methyl ester (l-NAME)

SUBMITTER: Trujillo AN 

PROVIDER: S-EPMC5668603 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature