Cryo-EM reconstruction of the chlororibosome to 3.2 A resolution within 24 h.

ABSTRACT: The introduction of direct detectors and the automation of data collection in cryo-EM have led to a surge in data, creating new opportunities for advancing computational processing. In particular, on-the-fly workflows that connect data collection with three-dimensional reconstruction would be valuable for more efficient use of cryo-EM and its application as a sample-screening tool. Here, accelerated on-the-fly analysis is reported with optimized organization of the data-processing tools, image acquisition and particle alignment that make it possible to reconstruct the three-dimensional density of the 70S chlororibosome to 3.2 Å resolution within 24 h of tissue harvesting. It is also shown that it is possible to achieve even faster processing at comparable quality by imposing some limits to

SUBMITTER: Forsberg BO 

PROVIDER: S-EPMC5668856 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature