Dataset Information

Xanthophylls lutein and zeaxanthin modify gene expression and induce synthesis of hyaluronan in keratinocyte model of human skin.

ABSTRACT:

Background

Clinical trials report benefits of the xanthophylls lutein and zeaxanthin for skin health. Here a keratinocyte culture was used to investigate the effects of in vitro xanthophyll treatment on gene expression and biochemical pathways.

Methods

We employed the EpiDerm tissue model, Affymetrix Human Genome Array U113, bioinformatics analyses, qPCR validation and biochemical assays for glycosaminoglycans.

Results

We discovered 176 genes were significantly (p<0.05) down-regulated (log 2FC>2) and 47 genes were significantly up-regulated. Among the down-regulated genes we validated by qPCR marked reduction in expression of peptidase inhibitors. Bioinformatic analysis of the up-regulated genes implicated biosynthetic pathways for glycosaminoglycans. We assayed but found no increase in production of sulfated glycosaminoglycans, however there was a significant increase in biosynthesis of hyaluronic acid, a non-sulfated glycan.

Conclusions

The pattern of xanthophyll-regulated genes and the resulting biochemical responses can be linked with the responses observed in clinic trials.

General significance

Skin health benefits from xanthophyll supplementation and this study reveals molecular mechanisms for some of the effects.

SUBMITTER: Li R

PROVIDER: S-EPMC5668877 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Publications

Xanthophylls lutein and zeaxanthin modify gene expression and induce synthesis of hyaluronan in keratinocyte model of human skin.

Li Rasia R Turner Stephen D SD Brautigan David L DL

Biochemistry and biophysics reports 20150821

<h4>Background</h4>Clinical trials report benefits of the xanthophylls lutein and zeaxanthin for skin health. Here a keratinocyte culture was used to investigate the effects of <i>in vitro</i> xanthophyll treatment on gene expression and biochemical pathways.<h4>Methods</h4>We employed the EpiDerm tissue model, Affymetrix Human Genome Array U113, bioinformatics analyses, qPCR validation and biochemical assays for glycosaminoglycans.<h4>Results</h4>We discovered 176 genes were significantly (<i>p ...[more]

PMID: 29124187

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Project description:PurposeTo evaluate macular pigment response to carotenoid supplementation in glaucomatous eyes.DesignDouble-masked, randomized, placebo-controlled clinical trial, the European Nutrition in Glaucoma Management Study (ClinicalTrials.gov identifier, NCT04460365).ParticipantsSixty-two participants (38 men, 24 women) with a diagnosis of open-angle glaucoma were enrolled. Forty-two were randomized to receive the active supplement, 20 participants were allocated to placebo.MethodsMacular pigment optical density (MPOD) was measured by autofluorescence using the Heidelberg Spectralis scanning laser ophthalmoscope. Macular pigment optical density volume within the central 6° of retinal eccentricity as well as MPOD at 0.23°, 0.51°, 0.74°, and 1.02° were recorded at baseline and at 6-month intervals over 18 months. Visual function was assessed using visual acuity, mesopic and photopic contrast sensitivity under glare conditions, photo stress recovery time, microperimetry, and Glaucoma Activities Limitation 9 questionnaire. Advanced glaucoma module scans of retinal nerve fiber layer thickness and ganglion cell complex thickness over the central 6° of retinal eccentricity also were completed at each study visit.Main outcome measuresChange in MPOD after supplementation with 10 mg lutein, 2 mg zeaxanthin, and 10 mg meso-zeaxanthin or placebo over 18 months.ResultsA mixed-model repeated measures analysis of variance revealed a statistically significant increase in MPOD volume (significant time effect: F(3,111) = 89.31, mean square error (MSE) = 1656.9; P < 0.01). Post hoc t tests revealed a significant difference in MPOD volume at each study visit for the treatment group (P < 0.01 for all), but no change in the placebo group (P > 0.05 for all). A statistically significant increase in mesopic contrast sensitivity under glare conditions was noted at 18 months in the treatment group, but not placebo. No other structural or functional changes were observed. No serious adverse events were noted during the trial.ConclusionsMacular pigment can be augmented in glaucomatous eyes by supplementation with a formulation containing the carotenoids lutein, zeaxanthin, and meso-zeaxanthin. The greatest relative benefit was observed in those with the lowest baseline levels, but increases were noted across all participants and each retinal eccentricity. The potential benefits of MP augmentation for macular health in glaucoma merit further long-term evaluation.

Dataset Information

Xanthophylls lutein and zeaxanthin modify gene expression and induce synthesis of hyaluronan in keratinocyte model of human skin.

Background

Methods

Results

Conclusions

General significance

Publications

Xanthophylls lutein and zeaxanthin modify gene expression and induce synthesis of hyaluronan in keratinocyte model of human skin.

Similar Datasets

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