Identification of <i>Spiroplasma</i><i>insolitum</i> symbionts in <i>Anopheles gambiae</i>.

ABSTRACT: Background: Insect symbionts have the potential to block the transmission of vector-borne diseases by their hosts. The advancement of a symbiont-based transmission blocking strategy for malaria requires the identification and study of Anopheles symbionts. Methods: High throughput 16S amplicon sequencing was used to profile the bacteria associated with Anopheles gambiae sensu lato and identify potential symbionts. The polymerase chain reaction (PCR) with specific primers were subsequently used to monitor symbiont prevalence in field populations, as well as symbiont transmission patterns. Results: We report the discovery of the bacterial symbiont, Spiroplasma, in Anopheles gambiae in Kenya. We determine that geographically dispersed Anophel

SUBMITTER: Chepkemoi ST 

PROVIDER: S-EPMC5668936 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature