Biosensor-aided high-throughput screening of hyper-producing cells for malonyl-CoA-derived products.

ABSTRACT:

Background

Malonyl-coenzyme A (CoA) is an important biosynthetic precursor in vivo. Although Escherichia coli is a useful organism for biosynthetic applications, its malonyl-CoA level is too low.

Results

To identify strains with the best potential for enhanced malonyl-CoA production, we developed a whole-cell biosensor for rapidly reporting intracellular malonyl-CoA concentrations. The biosensor was successfully applied as a high-throughput screening tool for identifying targets at a genome-wide scale that could be critical for improving the malonyl-CoA biosynthesis in vivo. The mutant strains selected synthesized significantly higher titers of the type III polyketide triacetic acid lactone (TAL), phloroglucinol, and free fatty acids compared to the wild-type strain, using m

SUBMITTER: Li H 

PROVIDER: S-EPMC5669015 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature