Tail-Cuff Technique and Its Influence on Central Blood Pressure in the Mouse.

ABSTRACT:

Background

Reliable measurement of blood pressure in conscious mice is essential in cardiovascular research. Telemetry, the "gold-standard" technique, is invasive and expensive and therefore tail-cuff, a noninvasive alternative, is widely used. However, tail-cuff requires handling and restraint during measurement, which may cause stress affecting blood pressure and undermining reliability of the results.

Methods and results

C57Bl/6J mice were implanted with radio-telemetry probes to investigate the effects of the steps of the tail-cuff technique on central blood pressure, heart rate, and temperature. This included comparison of handling techniques, operator's sex, habituation, and influence of hypertension induced by angiotensin II. Direct comparison of measurements obtained

SUBMITTER: Wilde E 

PROVIDER: S-EPMC5669161 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature