Protein Expression and Purification of the Hsp90-Cdc37-Cdk4 Kinase Complex from <i>Saccharomyces cerevisiae</i>.

ABSTRACT: Interactions between Hsp90, its co-chaperone Cdc37 and kinases have been biochemically studied for over three decades and have been shown to be functionally important in organisms from yeast to humans. However, formation of a stable complex for structural studies has been elusive. In this protocol we describe expression and purification of Hsp90-Cdc37-Cdk4 kinase protein complex from Saccharomyces cerevisiae utilizing the viral 2A sequences to titrate the three proteins at similar levels.

SUBMITTER: Verba KA 

PROVIDER: S-EPMC5669387 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature