Gene replacement and quantitative mass spectrometry approaches validate guanosine monophosphate synthetase as essential for <i>Mycobacterium tuberculosis</i> growth.

ABSTRACT: Guanosine monophosphate synthetase (GMPS), encoded by guaA gene, is a key enzyme for guanine nucleotide biosynthesis in Mycobacterium tuberculosis. The guaA gene from several bacterial pathogens has been shown to be involved in virulence; however, no information about the physiological effect of direct guaA deletion in M. tuberculosis has been described so far. Here, we demonstrated that the guaA gene is essential for M. tuberculosis H37Rv growth. The lethal phenotype of guaA gene disruption was avoided by insertion of a copy of the ortholog gene from Mycobacterium smegmatis, indicating that this GMPS protein is functional in M. tuberculosis. Protein validation of the guaA essentiality observed by PCR was approach

SUBMITTER: Villela AD 

PROVIDER: S-EPMC5669397 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature