Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection.

ABSTRACT:

Objective

To assess the circular RNAs (circRNAs) expression profile and explore the potential functions in human thoracic aortic dissection (TAD).

Methods

The differentially expressed circRNAs profiles of the aortic segments between human type A TAD patients (n=3) and age-matched normal donors (NA; n=3) were analyzed using the Arraystar human circRNAs microarray. Quantitative real-time PCR was used to validate the expression pattern of circRNAs, parental genes, and hsa-miR-320a; Western blotting confirmed MMP9 expression with additional samples. Bioinformatic tools including network analysis, Gene ontology, and KEGG pathway analysis were utilized.

Results

Among 8,173 detected circRNA genes, 156 upregulated and 106 downregulated significantly in human TAD

SUBMITTER: Zou M 

PROVIDER: S-EPMC5669851 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature