Development of a radiolabeled caninized anti-EGFR antibody for comparative oncology trials.

ABSTRACT: Due to large homology of human and canine EGFR, dogs suffering from spontaneous EGFR+ cancer can be considered as ideal translational models. Thereby, novel immunotherapeutic compounds can be developed for both human and veterinary patients. This study describes the radiolabeling of a canine anti-EGFR IgG antibody (can225IgG) with potential diagnostic and therapeutic value in comparative clinical settings. Can225IgG was functionalized with DTPA for subsequent chelation with the radionuclide ^99mTc. Successful coupling of 10 DTPA molecules per antibody on average was proven by significant mass increase in MALDI-TOF spectroscopy, gel electrophoresis and immunoblots. Following functionalization and radiolabeling, ^99mTc-DTPA-can225IgG fully retained its binding capacity to

SUBMITTER: Fazekas-Singer J 

PROVIDER: S-EPMC5669955 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature