Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation.

ABSTRACT: Type 2 diabetes mellitus (T2DM) is characterized by oxidative stress that could lead to chronic micro- and macrovascular complications. We hypothesized that some of the target organ damage is mediated by oxidative alterations in epigenetic mechanisms involving DNA methylation (5mC) and DNA hydroxymethylation (5hmC). We analyzed global DNA methylation and hydroxymethylation in peripheral blood cells in well-controlled and poorly controlled patients with T2DM and compared them with healthy controls. We also analyzed microarrays of DNA methylation and gene expression of other important tissues in the context of diabetes from the GEO database repository and then compared these results with our experimental gene expression data. DNA methylation and, more importantly, DNA hydroxymethylation leve

SUBMITTER: Pinzon-Cortes JA 

PROVIDER: S-EPMC5670276 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature