Construction and characterization of an improved DNA-launched infectious clone of duck hepatitis a virus type 1.

ABSTRACT:

Background

DNA-launched infectious system is a useful tool with high rescue efficiency that allows the introduction of mutations in specific positions to investigate the function of an individual viral element. Rescued virus particles could be harvested by directly transfecting the DNA-launched recombinant plasmid to the host cells, which will reduce labor and experimental cost by skipping the in vitro transcription assay.

Methods

A total of four overlapping fragments covering the entire viral genome were amplified and then were assembled into a transformation vector based on pIRES2-EGFP to establish the DNA-launched infectious system of duck hepatitis A virus type 1 (DHAV-1), named pIR-DHAV-1. Reverse transcription polymerase chain reaction (RT-PCR) detection, quantitative

SUBMITTER: Chen J 

PROVIDER: S-EPMC5670519 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature